Science

In Search of Putative FOXP3+ Cell Surface Markers

by Stephen Jones in 21.05.2009 19:15

Despite intense interest and scrutiny focused on FOXP3 as a key protein & master transcription factor, isolating and enriching for viable FOXP3 positive cells remains a challenge. Although cell separation/staining via CD4+CD25+ selection is commonly used, this technique has limited applications. Thus, surface markers specific for FOXP3 positive cells would be invaluable research tools as they would:

• Facilitate isolation & purification of viable

Treg cells

• Distinguish naturally occurring

CD4+CD25+cells from both naive and

recently activated CD4+CD25-

nonregulatory T cells

• Allow therapeutic manipulation of

Treg cells

As the search for putative FOXP3+ markers continue, Neuropilin-1, GPR83, & FR4 have emerged as potential candidates. IMGENEX is excited to offer a panel of flow cytometric characterized antibodies against:

• Neuropilin-1 (clone 211H6.01)

• GPR83 (polyclonal)

• Folate Receptor 4 (clones 12A5 & TH6)

Flow cytometric analysis of intracellular FOXP3 (IMG-5802D) and cell surface FR4 with clone 12A5 (IMG-6217C) (left) and clone TH6 (IMG-6218C) (right) at 0.06 ug/10^6 mouse splenocytes.

Flow cytometric analysis of Neuropilin-1 in CD4+CD25+ human PBMCs using A) an isotype control & B) DDX0440 at 0.5 ug/10^6 cells. These antibodies are available in multiple sizes and conjugates.

Resources:

IMGENEX India Pvt Ltd. the only biotech company in Orissa and one of its kinds in Eastern India. IMGENEX India started in Oct as an outsourcing branch of http://www.imgenex.com, San Diego, USA. Find out more information about FOXP3+ Cell Surface Markers.

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